Articles by "Screening"
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Original Article


SEROPOSITIVITY OF SYPHILIS OF THE PATIENTS VISITING GYNAECOLOGY, STI AND DERMATOLOGY CLINIC OF SULAV HOSPITAL, BELBARI, MORANG

Chaudhary DK, 1 Shah PK, 1 Khanal H 2
1Department of Microbiology, St. Xavier’s College, Maitighar, Kathmandu, 2Sulav Hospital, Belbari, Morang



ABSTRACT
Introduction: Syphilis is a sexually transmitted disease caused by T. pallidum subspecies Pallidum. The disease is divided into primary, secondary, and tertiary stages. Latent syphilis is the stage in which the infection performs a disappearing act of sorts.
Objective:    The main purpose of the study is to find out asymptomatic seroprevalence of syphilis from the blood samples.
Methods:     This Cross-sectional study was carried out in pathological lab of Sulav Hospital , Belbari, Morang between May 2011- October 2011. A total of 2139 blood samples of age 16-50 years patients were enrolled. Sera were screened by rapid plasma reagin (RPR) test kit. RPR reactive samples only were further tested by T. pallidum haemagglutination (TPHA) test kit.
Results:    From the study, overall syphilis seropositivity was 0.51%. The highest seropositivity of 0.74% (P-value 0.776) was found in age group 31-35 years with female predominant. Married & previous pregnant population had the highest seroprevalent of 1.21 (p-value 0.001) and 0.90% (p-value 0.266) respectively. Mongolian, Ethnic group, Secondary level literate population, abroad job seekers & returners population and patient with STIs history had the highest seroprevalent of 0.76% (p-value 0.52), 0.57% (p-value 0.977), 0.86% (p-value 0.728%) and 5.2% (p-value 0.001) respectively.
Conclusions: Study of seroprevalence of syphilis in asymptomatic cases is very important which help in the control of its consequence and prevent the further progression of syphilis.

Key words:    Asymptomatic, Latent stage, Rapid plasma reagin, Screening, Syphilis.





Incidence of Asymptomatic UTI & Antibiotics Susceptibility Pattern of Isolates in Primary School Going Children of Kathmandu Valley

Dhiraj Kr. Chaudhary  , Malavika Gupta,  Prabin Bhandari,  Reena Maharjan, Siyaram Bhagat



ABSTRACT

Urinary tract infection is one of the most common bacterial infections during childhood. It is caused by microbial invasion of tissue lining the urinary tract which extends from renal cortex to urethral meatus. Asymptomatic UTI is defined as a significant bacterial count (usually105organism/ml) present in the urine of a person without symptoms.
The main purpose of the study is to find out the incidence of asymptomatic UTI in primary school going children of different age groups & sex and to know the antibiotic susceptibility pattern of the isolates.
The study was carried out in Microbiology Laboratory of St. Xavier’s College, Maitighar, Kathmandu from February 6-March 21 ,2011. A total of 54 midstream urine sample of age 2-12 years children from different school of Kathmandu valley were collected & processed using standard microbiological techniques. All the samples were cultured on Blood agar and Macconkey agar to identify the potential pathogen. To observe Color, odor & turbidity, macroscopic observation was done. Microscopic examination was performed to observe pus cell, RBCs, epithelial cell, casts & crystals. Nitrate, protein, glucose, ketone & pH was determined by Uristrip analysis. The antibiotics sensitivity test was performed for those bacteria which were grown with significant number on culture. The colony count was performed & organisms were identified by biochemical tests.
From the study asymptomatic bacteriuria(UTI) was observed in 4 cases (7.4%)with female predominant out of total 54 samples. Among the isolates, Escherichia coli 2 (50%) was the predominant bacterial pathogen.
Study of incidence of asymptomatic UTI in primary school going children is very important in which Escherichia coli are found as major causative agents & most of the isolates were resistant to one or more antibiotics as tested in the study. 

Key words: Incidence, Asymptomatic UTI, Children, Screening, Culture, Antibiotics,Escherichia coli